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1 mg of RNase powder was added to 1 ml of freshly prepared 1X PBS solution to prepare a stock solution (1 mg/ml) which was kept at − 20 °C, 100 μl from the 1 mg/ml RNase A stock was added to 900 μl of 1X PBS (100 μg/ml working solution) and stored at 4 °C.Hoechst Whore
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