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In Mito, Im a woman dreaming of a man to hold close
About Myself
Come on in, I am Samantha, i am immersed in Mito culture. And Find A Prostitute is beyond awesome, you take my breath away. I am enthralled by Foot fetish and Anal Sex, my ideal match? Someone who makes me laugh and challenges me intellectually..
About Tokyo
So, we scarper, duck into an alley—dark, gritty, *Children of Men* dystopia shit. She’s giggles, I’m sweatin’, thinkin’ “This bird’s a riot!” She’s tellin’ me how prossies dodge the coppers—tip: wear a wig, switch it up! Genius, yeah? I’m happy now, buzzin’—Ruby’s a gem, a real dolly bird. “You’re my miracle, Kee!” I blurt, all dramatic-like, channelin’ that movie soul.
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Then, we decide to stroll through the park. Kairakuen Park is beautiful, especially in spring. Cherry blossoms everywhere! I’m feeling all poetic, like I should be writing haikus or something. But then, I trip over a root and almost faceplant. I’m like, “Not today, nature!”
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We describe a medium-throughput protocol to assess simultaneously mitophagy and mitochondrial mass by flow cytometry in vitro and in ex vivo retinal cultures and highlight the possibility of performing more complex assays and targeted screens by multiplexing with additional fluorescent probes! We present an optimized traditional and spectral flow cytometry protocol that provides sensitive simultaneous readouts of mitophagy and mitochondrial mass using tandem fluorescent reporters like mito-QC.Mito Brothel
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